Substance P- and GABA-like immunoreactivities are co-localized in axonal varicosities in the superficial laminae of cat but not rat spinal cord
Identifieur interne : 004003 ( Main/Exploration ); précédent : 004002; suivant : 004004Substance P- and GABA-like immunoreactivities are co-localized in axonal varicosities in the superficial laminae of cat but not rat spinal cord
Auteurs : Weiya Ma [Canada] ; A. Ribeiro-Da-Silva [Canada]Source :
- Brain Research [ 0006-8993 ] ; 1995.
English descriptors
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Abstract
Abstract: In the present study, we applied a combination of pre-embedding peroxidase-based immunocytochemistry and post-embedding immunogold staining to examine the synaptic interactions of substance P (SP) and γ-aminobutyric acid (GABA) in the superficial laminae of the dorsal horn of cat and rat spinal cord. We demonstrate for the first time the co-existence of SP and GABA immunoreactivities in axonal boutons in laminae I–III of cat spinal dorsal horn. In cat, most SP + GABA immunoreactive (IR) axonal boutons stablished synapses with SP-IR or non-IR dendrites. These synapses were exclusively symmetric. Quantitative analysis showed that the percentage of SP/GABA double labelled bouton profiles was higher (7%) in lamina I but was considerably lower in laminae IIo, IIi and III. Similarly, the density (number of bouton profiles per 100 μm2) of SP + GABA-IR bouton profiles was highest in lamina I. However, in agreement with previous studies, the co-localization of SP and GABA immunoreactivities was never detected in the rat dorsal horn. In both species, SP + GABA-IR or GABA-IR axonal bouton profiles were never seen presynaptic to SP-IR boutons. These findings provide a morphological basis for the interaction of excitatory and inhibitory agents in the nociceptive circuits in the dorsal horn of the cat and rat spinal cord.
Url:
DOI: 10.1016/0006-8993(95)00666-E
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Substance P- and GABA-like immunoreactivities are co-localized in axonal varicosities in the superficial laminae of cat but not rat spinal cord</title><author><name sortKey="Ma, Weiya" sort="Ma, Weiya" uniqKey="Ma W" first="Weiya" last="Ma">Weiya Ma</name></author><author><name sortKey="Ribeiro Da Silva, A" sort="Ribeiro Da Silva, A" uniqKey="Ribeiro Da Silva A" first="A." last="Ribeiro-Da-Silva">A. 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Ribeiro-Da-Silva</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, McGill University, 3655 Drummond Street, Room 1325, Montréal, Québec H3G 1Y6</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">692</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="99">99</biblScope><biblScope unit="page" to="110">110</biblScope></imprint><idno type="ISSN">0006-8993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Dorsal horn</term><term>Double labelling</term><term>Electron microscopy</term><term>Immunocytochemistry</term><term>Immunogold</term><term>Lamina I</term><term>Substantia gelatinosa</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In the present study, we applied a combination of pre-embedding peroxidase-based immunocytochemistry and post-embedding immunogold staining to examine the synaptic interactions of substance P (SP) and γ-aminobutyric acid (GABA) in the superficial laminae of the dorsal horn of cat and rat spinal cord. We demonstrate for the first time the co-existence of SP and GABA immunoreactivities in axonal boutons in laminae I–III of cat spinal dorsal horn. In cat, most SP + GABA immunoreactive (IR) axonal boutons stablished synapses with SP-IR or non-IR dendrites. These synapses were exclusively symmetric. Quantitative analysis showed that the percentage of SP/GABA double labelled bouton profiles was higher (7%) in lamina I but was considerably lower in laminae IIo, IIi and III. Similarly, the density (number of bouton profiles per 100 μm2) of SP + GABA-IR bouton profiles was highest in lamina I. However, in agreement with previous studies, the co-localization of SP and GABA immunoreactivities was never detected in the rat dorsal horn. In both species, SP + GABA-IR or GABA-IR axonal bouton profiles were never seen presynaptic to SP-IR boutons. These findings provide a morphological basis for the interaction of excitatory and inhibitory agents in the nociceptive circuits in the dorsal horn of the cat and rat spinal cord.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Québec</li></region><settlement><li>Montréal</li></settlement><orgName><li>Université McGill</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Ma, Weiya" sort="Ma, Weiya" uniqKey="Ma W" first="Weiya" last="Ma">Weiya Ma</name></region><name sortKey="Ribeiro Da Silva, A" sort="Ribeiro Da Silva, A" uniqKey="Ribeiro Da Silva A" first="A." last="Ribeiro-Da-Silva">A. Ribeiro-Da-Silva</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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